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Probabilistic methods for designing functional protein structures

Tuesday, January 30, 2024 - 10:30 to 11:30
Brian L. Trippe, Postdoctoral Research Fellow, Department of Statistics, Columbia University
Statistics Seminar
ESB 4192 / Zoom

To join this seminar virtually: Please request Zoom connection details from ea [at] stat.ubc.ca

Abstract: The biochemical functions of proteins, such as catalyzing a chemical reaction or binding to a virus, are typically conferred by the geometry of only a handful of atoms.  This arrangement of atoms, known as a motif, is structurally supported by the rest of the protein, referred to as a scaffold.  A central task in protein design is to identify a diverse set of stabilizing scaffolds to support a motif known or theorized to confer function. This long-standing challenge is known as the motif-scaffolding problem.

In this talk, I describe a statistical approach I have developed to address the motif-scaffolding problem.  My approach involves (1) estimating a distribution supported on realizable protein structures and (2) sampling scaffolds from this distribution conditioned on a motif.  For step (1) I adapt diffusion generative models to fit example protein structures from nature.  For step (2) I develop sequential Monte Carlo algorithms to sample from the conditional distributions of these models.  I finally describe how, with experimental and computational collaborators, I have generalized and scaled this approach to generate and experimentally validate hundreds of proteins with various functional specifications.